panc 1  (ATCC)

Journal: International Journal of Oncology

Article Title: Circ72309 modulates gemcitabine metabolism and gemcitabine sensitivity in pancreatic cancer: Serum Circ72309 levels as a potential predictor of treatment response

doi: 10.3892/ijo.2026.5849

Figure Lengend Snippet: Effects of circ72309 overexpression on cellular function in gemcitabine-resistant cell lines. (A) Vector schema for overexpression of circ72309. (B) Dose-response curves of gemcitabine in GR_NC and GR_circ72309OE clones. GR_circ72309OE clones showed a concentration-dependent decrease in viability with a typical sigmoidal fitting (IC 50 values: GR3, 13.3 ng/ml, R 2 =0.974; GR8, 130 ng/ml, R 2 =0.951; GR10, 16.9 ng/ml, R 2 =0.984), whereas GR_NC cells retained high viability even at maximal gemcitabine concentrations, consistent with the resistant phenotype. Fitted curves are shown as solid lines, while resistant clone values without a typical sigmoidal decline are represented as dashed lines. (C) MTT assays were used to assess the effects of si-circ72309 in PC cell lines (MIAPaCa-2, PSN-1 and Panc-1). Dose-response curves were analyzed using non-linear regression with a four-parameter logistic model; all fitted curves showed excellent agreement with the experimental data (R 2 >0.95). Fitted curves are shown as solid lines. (D) Apoptosis assays following treatment with 20 ng/ml gemcitabine. * P<0.05. circRNA, circular RNA; PC, pancreatic cancer; GR, gemcitabine resistant; NC, negative control; siRNA, small interfering RNA.

Article Snippet: In the present study, three human PC cell lines: MIAPaCa-2 (RRID: CVCL_0428) purchased from the Japan Cancer Research Resources Bank, Panc-1 (RRID: CVCL_0480) purchased from the American Type Culture Collection and PSN-1 (RRID: CVCL_1644) obtained from the European Collection of Authenticated Cell Culture.

Techniques: Over Expression, Cell Function Assay, Plasmid Preparation, Clone Assay, Concentration Assay, Negative Control, Small Interfering RNA

Journal: bioRxiv

Article Title: Developing potent and selective TBK1 molecular glue degraders for cancer immunotherapy

doi: 10.64898/2026.01.30.702304

Figure Lengend Snippet: (A) Western blot analysis of total TBK1 and phosphorylated TBK1 (p-TBK1) in PANC-1 cells treated with the TBK1 molecular glue CCT412020 (1 µM) in the presence or absence of TNF and/or IFNγ. (B) Cell viability assay in PANC-1 cells treated with the TBK1 molecular glue CCT412020 (1 µM) in the presence or absence of TNF (10 ng/ml)/IFNγ (5 ng/ml). Cells were treated for 48h, and viability was quantified following treatment. (C) Cell viability assay in MC38 hCRBN cells treated with TNF and the pan-caspase inhibitor emricasan to induce necroptosis, in the presence of either the TBK1 molecular glue CCT412020 (1 µM). Cell viability was quantified following treatment as indicated.

Article Snippet: MCF-7, HCC38, BT-549, MDA-MB-231, MDA-MB-468, HeLa, and PANC-1 cell lines were obtained from ATCC.

Techniques: Western Blot, Viability Assay

Journal: bioRxiv

Article Title: A syndecan-based genetic approach to coat the surface of small extracellular vesicles with Nanobodies

doi: 10.64898/2026.01.29.702589

Figure Lengend Snippet: (A) Dose-response curve illustrating the uptake of non-coated CCM sEVs loaded with Nanoluciferase (NL)-syntenin. Panc-1 recipient cells were incubated for 4 h with increasing doses of CCM sEVs. (Y-axis) Luminescence signals (relative light units, RLU) were detected 5 min after washing Panc-1 cells, using the membrane permeant substrate Furimazine. (X-axis) Doses are expressed as the number of plated producing HEK293 cells per single plated Panc-1 recipient cell (p/r). Note the saturation of uptake from 100 p/r on. (B) Representative Western blot of the cell lysates (CL), large EV (lEV) and small EV (sEV) fractions (obtained by dUC) of cNef or cEGFR cells cultured in the absence of serum. Note that both chimeras are efficiently sorted into sEVs. Syntenin is used as sEV marker. Mock refers to HEK293 cells stably transfected with an empty vector. CL corresponds to 20.000 cells. EVs were collected from the conditioned media of 3.6 x 10 6 cells. Ponceau red was used as loading and transfer control. All samples were loaded on the same membrane. For uncropped blot see Fig. S7. (C) Schematic representation of CCM sEVs coated with different Nb and loaded with NL-syntenin used for uptake experiments. Panc-1 cells were incubated with CCM sEVs under saturating doses (200:1 p/r). Inset: Representative confocal micrographs of Panc-1 cells illustrating their EGFR expression (grey). Nuclei are stained with DAPI (blue). Scale bar is 50 µm. (D) Time course of luminescence signals (y-axis, RLU) detected in Panc-1 cells following incubation with the different CCM sEVs as shown in C . Note that anti-EGFR CCM sEVs exhibit a significant increase in uptake compared to non-coated or anti-Nef CCM sEVs. Bars represent mean values + SEM. Statistical significance was determined by comparing the area under the curve using one-way ANOVA test with Tukey’s correction. Related to Fig. S6 and S7 .

Article Snippet: HEK293, and Panc-1 cells (ATCC) were cultured in DMEM supplemented with 10% FBS.

Techniques: Incubation, Membrane, Western Blot, Cell Culture, Marker, Stable Transfection, Transfection, Plasmid Preparation, Control, Expressing, Staining

Journal: Oncology Reports

Article Title: Prognostic significance of fibroblast senescence and senescence-associated secretory phenotype factor expression in the tumor microenvironment of pancreatic ductal adenocarcinoma

doi: 10.3892/or.2025.9031

Figure Lengend Snippet: Evaluation of p16- and IL-6-positive cells in the stroma of pancreatic ductal adenocarcinoma. (A) Representative immunofluorescence staining of a pancreatic cancer resection specimen (αSMA, green; p16, red; DAPI, blue; scale bar, 50 and 25 µm). In the pancreatic cancer stroma, pancreatic fibroblasts (αSMA-positive) expressing the senescence marker p16 were identified (yellow arrows). (B) Representative immunofluorescence staining of a pancreatic cancer resection specimen (p16, red; IL-6, green; DAPI, blue; scale bar, 100 and 25 µm). Fibroblasts positive for p16 and the senescence-associated secretory phenotype factor IL-6 were observed in the pancreatic cancer stroma (yellow arrows). (C) A significant positive correlation between the expression intensity of p16- and IL-6-positive fibroblasts in the pancreatic cancer stroma (Pearson's correlation coefficient, r=0.585; P<0.0001).

Article Snippet: The Panc-1 cell line, derived from human pancreatic cancer cells (cat. no. CRL-1469; American Type Culture Collection) was used in the present study.

Techniques: Immunofluorescence, Staining, Expressing, Marker

Journal: Oncology Reports

Article Title: Prognostic significance of fibroblast senescence and senescence-associated secretory phenotype factor expression in the tumor microenvironment of pancreatic ductal adenocarcinoma

doi: 10.3892/or.2025.9031

Figure Lengend Snippet: Relationship between IL-6 expression intensity in the pancreatic ductal adenocarcinoma stroma and prognosis after pancreaticoduodenectomy. (A) A significant negative correlation between the expression intensity of IL-6-positive fibroblasts in the pancreatic cancer stroma and overall survival (Pearson's correlation coefficient, r=−0.336; P=0.0012). (B) A receiver operating characteristic curve based on the expression intensity of IL-6-positive fibroblasts in the pancreatic cancer stroma and overall survival (AUC=0.754, sensitivity=0.736, specificity=0.734). The IL-6 H-score cut-off value was 179; patients with H-scores <179 were defined as the IL-6 low expression group, and those with scores ≥179 as the IL-6 high expression group. (C) Survival curves for patients with pancreatic cancer with low (solid line) and high (dotted line) -IL-6 expression (log-rank test, P=0.00002). Representative pancreatic cancer specimen with (D) weak, (E) moderate and (F) strong IL-6 positivity (scale bar, 100 µm). (G) Non-tumorous pancreatic specimen (scale bar, 100 µm).

Article Snippet: The Panc-1 cell line, derived from human pancreatic cancer cells (cat. no. CRL-1469; American Type Culture Collection) was used in the present study.

Techniques: Expressing

Journal: Oncology Reports

Article Title: Prognostic significance of fibroblast senescence and senescence-associated secretory phenotype factor expression in the tumor microenvironment of pancreatic ductal adenocarcinoma

doi: 10.3892/or.2025.9031

Figure Lengend Snippet: Establishment of human pancreatic fibroblast cell lines and induction of cellular senescence and the senescence-associated secretory phenotype. (A) Pancreatic fibroblasts established from resected pancreatic specimens. Cells showed a spindle-shaped morphology with cytoplasmic protrusions (AE1/3-negative, αSMA- and vimentin-positive; scale bar, 10 µm). (B) SA-β-gal staining of IR pancreatic fibroblasts (10 Gy) increased over time (scale bar, 50 µm). (C) SA-β-gal staining of pancreatic fibroblasts 9 days increased with gradient irradiation (3, 6 and 10 Gy; scale bar, 50 µm). (D) Western blot analysis of p16 protein expression in IR pancreatic fibroblasts (10 Gy X-rays on days 0, 3, 6 and 9) showed increased p16 protein expression from day 3 onward compared with the non-IR group. (E) Western blot analysis showed a gradual increase of IL-6 protein expression from day 3 onwards to day 9 post-irradiation. (F) Reverse transcription-quantitative PCR confirmed increased IL-6 mRNA expression after irradiation over 9 days of culture. Data are presented as mean ± SD (n=6). (G) Immunofluorescence of pancreatic fibroblasts. IL-6 expression was increased on day 9 (IL-6, red; DAPI, blue; scale bar, 50 µm). *P<0.01 and **P<0.001. ns, not significant; H&E, hemoxylin and eosin; SA-β-gal, senescence-associated β-galactosidase; aSMA, a smooth muscle actin; ACTB, β-actin; IR, irradiated.

Article Snippet: The Panc-1 cell line, derived from human pancreatic cancer cells (cat. no. CRL-1469; American Type Culture Collection) was used in the present study.

Techniques: Staining, Irradiation, Western Blot, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Immunofluorescence

Journal: Oncology Reports

Article Title: Prognostic significance of fibroblast senescence and senescence-associated secretory phenotype factor expression in the tumor microenvironment of pancreatic ductal adenocarcinoma

doi: 10.3892/or.2025.9031

Figure Lengend Snippet: Co-culture experiments of Panc-1 cells with IR human pancreatic fibroblasts or their culture supernatants. (A) Representative images of pancreatic fibroblasts (10 Gy IR or non-IR) that were co-cultured with Panc-1 cells (scale bar, 100 µm), and (B) the number of cells that invaded through the gel and membrane was counted (n=6). (C) A confluent monolayer of Panc-1 cells was scratched with a pipette, and the culture supernatant of IR or non-IR pancreatic fibroblasts was added (scale bar, 100 µm). (D) After 12 h, the area of cell migration was measured (n=6). *P<0.05 and **P<0.01. (E) Panc-1 cells were cultured with culture supernatants of IR or non-IR pancreatic fibroblasts, and proliferation was assessed after 24 and 48 h using the MTT assay (n=8). Data are presented as mean ± SD. *P<0.05, **P<0.01 and ***P<0.001. aSMA, a smooth muscle actin; Ctrl, control; IR, irradiated.

Article Snippet: The Panc-1 cell line, derived from human pancreatic cancer cells (cat. no. CRL-1469; American Type Culture Collection) was used in the present study.

Techniques: Co-Culture Assay, Cell Culture, Membrane, Transferring, Migration, MTT Assay, Control, Irradiation

Journal: The Journal of Biological Chemistry

Article Title: KRAS G12D mutation promotes pancreatic tumorigenesis by suppressing sirtuin three via the guanine nucleotide exchange factor RCC1

doi: 10.1016/j.jbc.2025.111057

Figure Lengend Snippet: SIRT3 suppresses pancreatic cancer growth in vitro and in vivo . A, kaplan-meier survival plot of pancreatic cancer patients with high and low SIRT3 expression at 75%/25% cutoff from The Cancer Genome Atlas database. B, SIRT3 protein levels in SIRT3 overexpressed (SIRT3 OE) and control (Vector) PANC-1 cells. C, growth curves of PANC-1 Vector cells and PANC-1 SIRT3 OE cells in vitro . The data are presented as mean ± SD ( n = 3). p values were determined by Student’s t test. ∗∗, p < 0.01. D, SIRT3 protein levels in SIRT3 knockdown (SIRT3 sh1, sh2) and control (NC) PANC-1 cells. E, growth curves of PANC-1 NC, SIRT3 sh1 and sh2 cells in vitro . The data are presented as mean ± SD ( n = 3). p values were determined by Student’s t test. ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001. F, schematic illustration of study design to evaluate the impact of SIRT3 on PANC1 tumor growth in vivo . G, growth curve of SIRT3 overexpressed (SIRT3 OE) and control (Vector) PANC1 subcutaneous tumors. The data are presented as mean ± SD ( n = 5). p values were determined by Student’s t test. ∗∗, p < 0.01. H , PANC1 subcutaneous tumors of the SIRT3 overexpression and control group. The characters at the top of the photo represent the mouse number. The length of the scale bar represents 1 cm. I, SIRT3 overexpressed and the control PANC1 tumor weight. The red and blue points connected by the line represent tumors inoculated on each side of the same mouse. p values were determined by Student’s t test. ∗∗, p < 0.01. J, SIRT3 immunohistochemistry of the SIRT3 overexpressed and the control PANC1 subcutaneous tumors. The images are representative sections of tumor #2 shown in A , which contains the IHC images of all tumors including the same source images shown here. The length of the scale bar represents 100 μm. K, Growth curve of SIRT3 knockdown (SIRT3 sh1, sh2) and control (NC) PANC1 subcutaneous tumors. The data are presented as mean ± SD ( n = 5). p values were determined by Student’s t test. ∗∗, p < 0.01; ∗∗∗, p < 0.001. L, PANC1 subcutaneous tumors of the SIRT3 knockdown and control group. M, SIRT3 knockdown and the control PANC1 tumor weight. The data are presented as mean ± SD ( n = 5). p values were determined by Student’s t test. ∗∗, p < 0.01; ∗∗∗∗, p < 0.0001. N, SIRT3 immunohistochemistry of the SIRT3 knockdown and the control PANC1 subcutaneous tumors. The images are representative sections of tumors #2 and #3 in B , which contains the IHC images of all tumors including the same source images shown here. The length of the scale bar represents 100 μm.

Article Snippet: hTERT-HPNE, HEK293, HEK293T PANC-1 and AsPC-1 cells were purchased from ATCC.

Techniques: In Vitro, In Vivo, Expressing, Control, Plasmid Preparation, Knockdown, Over Expression, Immunohistochemistry